Unit:
Κατεύθυνση Κλινική Βιοχημεία - Μοριακή ΔιαγνωστικήLibrary of the School of Science
Supervisors info:
Διδώ Βασιλακοπούλου, Αναπληρώτρια Καθηγήτρια Τμήμα Βιολογίας, ΕΚΠΑ,
Ανδρέας Σκορίλας Καθηγητής Τμήμα Βιολογίας, ΕΚΠΑ
Original Title:
ΜΕΛΕΤΗ ΤΗΣ ΣΥΣΧΕΤΙΣΗΣ ΤΗΣ ΕΚΦΡΑΣΗΣ ΤΗΣ L- DOPA ΑΠΟΚΑΡΒΟΞΥΛΑΣΗΣ ΜΕ ΤΗΝ ΟΞΕΙΔΩΤΙΚΗ ΚΑΤΑΠΟΝΗΣΗ
Translated title:
Study of association of the L-DOPA decarboxylase expression with oxidative stress
Summary:
Parkinson's disease is the second most common neurodegenerative disease worldwide and is the second most common cause of dementia after Alzheimer's disease, affecting about 2% of the population over 65 years old. The pathophysiology of the disease is mainly located in the compact part of the substantia nigra, where there is progressive degeneration of dopaminergic neurons, which produce the neurotransmitter dopamine at their synapses. A significant cause of the disease is oxidative stress on dopaminergic neurons, caused by the production of reactive oxygen and nitrogen species. The main metabolite of oxidative stress is H2O2, which was extensively studied in this thesis and causes damage to proteins, lipids, and DNA. On the other hand, ascorbic acid is a potent antioxidant that participates in the CNS balance. The study aimed to correlate L-DOPA decarboxylase (DDC) expression with oxidative stress. DDC catalyzes the decarboxylation of L-DOPA, a precursor molecule of dopamine, to form dopamine and 5-hydroxytryptophan to produce serotonin. Two cellular lines, CHO-K1 (Chinese Hamster Ovary) and CHO-DDC, were used for the experimental procedure. Cell viability and apoptosis studies in both oxidative and antioxidant environments showed the protective effect of human DDC against cell death under high oxidation conditions and toxic concentrations of antioxidant factors. Western blot analyses confirmed these specific results with increased protein expression of the molecule. In conditions of H2O2 and ascorbic acid administration, the reversal of cell death was observed, making DDC a molecule of utmost importance for cell death.
Main subject category:
Science
Keywords:
L-DOPA decarboxylase, oxidative stress, apoptosis, cell death, Western blotting, diphenylamine reaction, cell lines
Number of references:
243
File:
File access is restricted until 2027-04-29.
ΜΕΛΕΤΗ ΤΗΣ ΣΥΣΧΕΤΙΣΗΣ ΤΗΣ ΕΚΦΡΑΣΗΣ ΤΗΣ L-DOPA ΑΠΟΚΑΡΒΟΞΥΛΑΣΗΣ ΜΕ ΤΗΝ ΟΞΕΙΔΩΤΙΚΗ ΚΑΤΑΠΟΝΗΣΗ.pdf
2 MB
File access is restricted until 2027-04-29.
