Unit:
Κατεύθυνση Κλινική Βιοχημεία - Μοριακή ΔιαγνωστικήLibrary of the School of Science
Supervisors info:
Ευρύκλεια Λιανίδου, Καθηγήτρια Τμήμα Χημείας ΕΚΠΑ,
Αθηνά Μάρκου, Επίκουρη Καθηγήτρια Τμήμα Χημείας ΕΚΠΑ,
Αμάντα Ψυρρή, Καθηγήτρια Τμήμα Ιατρικής ΕΚΠΑ
Original Title:
Υγρή Βιοψία: Μελέτη επιγενετικών δεικτών στο cell-free DNA ασθενών με καρκίνο του παχέος εντέρου
Translated title:
Liquid Biopsy: Study of epigenetic markers in cell-free DNA of colorectal cancer patients
Summary:
Colorectal cancer (CRC) is the third most common type of cancer with 1,931,590 new cases and second in mortality with 935,173 worldwide for the year 2020. It is estimated that the number of new CRC cases will increase by 63% while the mortality will increase by 73% by 2040. Applications of liquid biopsy in CRC are expected to provide substantial solutions in patient management.
Epigenetic modifications consist one of the major hallmarks of cancer. DNA methylation is the most important and well-studied epigenetic mechanism in cancer. In CRC, DNA methylation has been found to be involved in the pathways of carcinogenesis. 17% of sporadic CRCs show the CpG Island Methylator Phenotype (CIMP), which is characterized by the hypermethylation of many genes at CpG islands in their promoter region. Recently, the first plasma cell-free DNA (cfDNA) test for population screening for CRC (Epi proColon AG, Germany), which detects methylation in the SEPT9 gene promoter, was approved by the FDA.
In the present study, we studied promoter methylation of the WIF-1, APC and RASSF1A genes in plasma-cfDNA samples from CRC patients and correlated our findings with the levels of the cancer markers CEA and CA 19-9 in blood serum and with the MSI status of the patients. In total, 42 plasma cfDNA samples, 26 preoperative and 16 postoperative from 28 CRC patients, were examined. In addition, serum samples from the 28 patients were analyzed to determine the tumor markers CEA and CA 19-9 before and after surgery.
Main subject category:
Science
Other subject categories:
Health Sciences
Keywords:
DNA methylation, APC, RASSF1A, WIF-1, Colorectal cancer
Number of references:
333
File:
File access is restricted until 2027-04-22.
Tsangari_Maria_MSc.pdf.pdf
3 MB
File access is restricted until 2027-04-22.
